From: Oral cavity swabbing for diagnosis of group a Streptococcus: a prospective study
Research and year of publication | Population studied | Sample size | Site of detection | Method of detection | Sensitivity | Reference standard |
---|---|---|---|---|---|---|
Brien et al. 1985 | Children | 12 | 9 different areas in the oral cavity | Cultures were inoculated on DSBA. Plates were incubated for 18–24 h at 37 °C in 10% CO2. | No sensitivity, specificity calculations. 63% of cultures from unacceptable sites showed some growth. | Tonsils and posterior pharyngeal wall |
Gunn et al. 1985 | children | 20 | 7 different areas in the oral cavity | Cultures were inoculated on DBSA and on DBSA-SXT. Plates were incubated for 18–24 h at 35 °C in 5% CO2 in air. | No sensitivity, specificity calculations. Recovery of GAS from optimal vs. unsatisfactory sites were 53% vs. 24 and 75% vs. 42% on DBSA and DBSA-SXT respectively. | Tonsils, posterior pharynx and posterior tongue. |
Fox et al. 2006 | children | 53 | 2 swabs (double swab collection) **throat swab (posterior pharynx and tonsils) **mouth swab (tongue and buccal mucosa) | **RADT (Abbott Signify Rapid Strep A test) **a DNA probe (a nucleic acid probe test) after 24 h **inoculation on DSBA-SXT (Becton Dickinson) in 5% CO2 at 35 °C for 48 h. | RADT – 19.4% (7.5–37.5%) DNA probe – 41.9% (23.9–60.9%) Culture (48 h) – 80.6% (62.5–92.5%) | positive culture or DNA probe of posterior pharynx/tonsils |
Kelly L 2007 | Children and adults | 64 | 2 swabs **throat swab (posterior pharynx and tonsils) **buccal mucosa | Both swabs were tested using RADT (The SureStep Strep A (II) Test by Applied Biotech) | Sensitivity of mouth culture was 5.6%. | RADT from optimal sites. |