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Table 2 Studies conducted comparing mouth and throat cultures

From: Oral cavity swabbing for diagnosis of group a Streptococcus: a prospective study

Research and year of publicationPopulation studiedSample sizeSite of detectionMethod of detectionSensitivityReference standard
Brien et al. 1985Children129 different areas in the oral cavityCultures were inoculated on DSBA. Plates were incubated for 18–24 h at 37 °C in 10% CO2.No sensitivity, specificity calculations.
63% of cultures from unacceptable sites showed some growth.
Tonsils and posterior pharyngeal wall
Gunn et al. 1985children207 different areas in the oral cavityCultures were inoculated on DBSA and on DBSA-SXT. Plates were incubated for 18–24 h at 35 °C in 5% CO2 in air.No sensitivity, specificity calculations.
Recovery of GAS from optimal vs. unsatisfactory sites were 53% vs. 24 and 75% vs. 42% on DBSA and DBSA-SXT respectively.
Tonsils, posterior pharynx and posterior tongue.
Fox et al. 2006children532 swabs (double swab collection)
**throat swab (posterior pharynx and tonsils)
**mouth swab (tongue and buccal mucosa)
**RADT (Abbott Signify Rapid Strep A test)
**a DNA probe (a nucleic acid probe test) after 24 h **inoculation on DSBA-SXT (Becton Dickinson) in 5% CO2 at 35 °C for 48 h.
RADT –
19.4% (7.5–37.5%)
DNA probe –
41.9% (23.9–60.9%)
Culture (48 h) –
80.6% (62.5–92.5%)
positive culture or DNA probe of posterior pharynx/tonsils
Kelly L 2007Children and adults642 swabs
**throat swab (posterior pharynx and tonsils)
**buccal mucosa
Both swabs were tested using RADT (The SureStep Strep A (II) Test by Applied Biotech)Sensitivity of mouth culture was 5.6%.RADT from optimal sites.
  1. DSBA 5% defibrinated sheep blood agar, DSBA-SXT 5% defibrinated sheep blood agar supplemented with sulfamethoxazole and trimetophrim, RADT rapid antigen detection test